Journal: Antimicrobial Agents and Chemotherapy

Article Title: Activities of Combinations of Antistaphylococcal Antibiotics with Fusidic Acid against Staphylococcal Biofilms in In Vitro Static and Dynamic Models

doi: 10.1128/AAC.00598-18

Figure Lengend Snippet: Origin and antibiotic susceptibility of the strains under study

Article Snippet: Antibiofilm activity was then evaluated against the reference strain ATCC 25923 and the clinical isolate 80224422456 ( ).

Techniques: Infection

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Activities of Combinations of Antistaphylococcal Antibiotics with Fusidic Acid against Staphylococcal Biofilms in In Vitro Static and Dynamic Models

doi: 10.1128/AAC.00598-18

Figure Lengend Snippet: Activity of fusidic acid (FUS) and daptomycin (DAP), linezolid (LZD), vancomycin (VAN), moxifloxacin (MXF), rifampin (RIF), or doxycycline (DOX) alone or in combination with fusidic acid after 48 h of incubation with biofilms from ATCC 25923 and 5 clinical isolates. The graph shows the reduction in bacterial viability evaluated by the percentage reduction in resorufin fluorescence compared to that of the untreated control. Antibiotics are all used at their human fCmax values (see Table 2). Each symbol corresponds to a bacterial strain, with the mean reduction and standard deviation appearing as black horizontal lines. Statistical analysis was by one-way analysis of variance (ANOVA) with Tukey's post hoc test. #, highlights combinations for which the mean reduction was higher than that observed for the drugs alone. See Table 3 for individual data of each strain.

Article Snippet: Antibiofilm activity was then evaluated against the reference strain ATCC 25923 and the clinical isolate 80224422456 ( ).

Techniques: Activity Assay, Incubation, Fluorescence, Standard Deviation

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Activities of Combinations of Antistaphylococcal Antibiotics with Fusidic Acid against Staphylococcal Biofilms in In Vitro Static and Dynamic Models

doi: 10.1128/AAC.00598-18

Figure Lengend Snippet: Activity of fusidic acid alone or combined with antistaphylococcal antibiotics against biofilms of the reference strain ATCC 25923 and 5 clinical isolates grown in microtiter plates

Article Snippet: Antibiofilm activity was then evaluated against the reference strain ATCC 25923 and the clinical isolate 80224422456 ( ).

Techniques: Activity Assay, Fluorescence

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Activities of Combinations of Antistaphylococcal Antibiotics with Fusidic Acid against Staphylococcal Biofilms in In Vitro Static and Dynamic Models

doi: 10.1128/AAC.00598-18

Figure Lengend Snippet: Activity of fusidic acid (FUS) alone or combined with selected antibiotics (daptomycin [DAP], linezolid [LZD], and vancomycin [VAN]) after 48 h of incubation with biofilms from ATCC 25923. The graphs show the residual viability evaluated by the reduction in resorufin fluorescence compared to that of the untreated control (CT) as a function of the concentration of fusidic acid. Combined antibiotics were added at a fixed concentration corresponding to their human fCmin (top) or fCmax (bottom). The horizontal, colored dotted line in each panel shows the effect of the combined antibiotic used alone at the selected concentration; therefore, all data points located in the colored area correspond to activities higher than that measured for this combined antibiotic alone. The black vertical dotted lines show the fCmin and fCmax of fusidic acid. All data are the means ± SDs from quadruplicates.

Article Snippet: Antibiofilm activity was then evaluated against the reference strain ATCC 25923 and the clinical isolate 80224422456 ( ).

Techniques: Activity Assay, Incubation, Fluorescence, Concentration Assay

Journal: Antimicrobial Agents and Chemotherapy

Article Title: Activities of Combinations of Antistaphylococcal Antibiotics with Fusidic Acid against Staphylococcal Biofilms in In Vitro Static and Dynamic Models

doi: 10.1128/AAC.00598-18

Figure Lengend Snippet: Activity of fusidic acid (FUS) alone or combined with daptomycin (DAP), linezolid (LZD), or vancomycin (VAN) against biofilms from the reference strain ATCC 25923 (top) or the clinical isolate 80224422456 (bottom) in the CDC bioreactor. FUS, LZD, and VAN were injected twice (0 h and 12 h), and DAP once (0 h), at the following concentrations (to mimic fCmax): FUS, 14 mg/liter; DAP, 9.8 mg/liter; LZD, 17 mg/liter; VAN, 20 mg/liter. Peristaltic pumps were then started to infuse antibiotic-free medium at rate set up to mimic a t1/2 of 12 h for FUS, 8 h for DAP, and 6 h for LZD and VAN.

Article Snippet: Antibiofilm activity was then evaluated against the reference strain ATCC 25923 and the clinical isolate 80224422456 ( ).

Techniques: Activity Assay, Injection